A Germ Line Specific DNA Sequence is Transcribed in Tetrahymena
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In the ciliated protozoan Tetrahymena 10–20% of the DNA sequences are micronucleus (germ line) specific. Six members of a family of repeated mic-specific DNA sequences are homologous to a 1.5-kb poly(A)+ RNA. The transcript is present in mature cells starved in 10 or 60 mM Tris, in starved immature cells, and in stationary cells. RNA from log-phase and heat-shocked cells does not have detectable levels of the transcript. These data indicate that at least one germ line limited DNA sequence is transcribed in the micronucleus of Tetrahymena.