Title

A Germ Line Specific DNA Sequence is Transcribed in Tetrahymena

Document Type

Article

Language

eng

Format of Original

11 p.

Publication Date

3-1987

Publisher

Elsevier

Source Publication

Developmental Biology

Source ISSN

0012-1606

Abstract

In the ciliated protozoan Tetrahymena 10–20% of the DNA sequences are micronucleus (germ line) specific. Six members of a family of repeated mic-specific DNA sequences are homologous to a 1.5-kb poly(A)+ RNA. The transcript is present in mature cells starved in 10 or 60 mM Tris, in starved immature cells, and in stationary cells. RNA from log-phase and heat-shocked cells does not have detectable levels of the transcript. These data indicate that at least one germ line limited DNA sequence is transcribed in the micronucleus of Tetrahymena.

Comments

Developmental Biology, Vol. 120, No. 1 (March 1987): 259-269. DOI.

Kathleen Karrer was affiliated with Brandeis University at the time of publication.