Position Effect for Adenine Methylation in the Macronuclear DNA of Tetrahymena

Authors

Teresa A. Van Nuland, Marquette University
Elizabeth E. Capowski, Fred Hutchinson Cancer Research CenterFollow
Kathleen M. Karrer, Marquette UniversityFollow

Document Type

Article

Language

eng

Format of Original

3 p.

Publication Date

5-19-1995

Publisher

Elsevier

Source Publication

Gene

Source ISSN

0378-1119

Abstract

The macronucleus of the ciliate Tetrahymena contains about 45 copies of the genome. The fraction of the molecules on which an adenine residue is modified to N⁶-methyladenine is characteristic of the specific site, and consistent between clones. A fragment of DNA containing a site that is uniformly methylated on the macronuclear chromosome was moved to a new location on the extrachromosomal rDNA. The methylation pattern of the fragment on the rDNA was different from that on the chromosome. The data suggest that DNA sequence is not sufficient to determine the level of methylation.

Comments

Gene, Vol. 157, No. 1--2 (May 19, 1995): 235-237. DOI.

Recommended Citation

Van Nuland, Teresa A.; Capowski, Elizabeth E.; and Karrer, Kathleen M., "Position Effect for Adenine Methylation in the Macronuclear DNA of Tetrahymena" (1995). Biological Sciences Faculty Research and Publications. 178.
https://epublications.marquette.edu/bio_fac/178