Document Type




Format of Original

7 p.

Publication Date



Society for Neuroscience

Source Publication

Journal of Neuroscience

Source ISSN



When expressed in the Xenopus oocyte, the minK protein induces a slowly activating voltage-dependent potassium current (Isk). We studied the modulation of this current by altering intracellular cAMP levels and found that the amplitude of Isk is dramatically increased by treatments that raise cAMP levels and decreased by agents that lower cAMP levels. Preinjection of a protein inhibitor of the cAMP-dependent protein kinase blocked the effects of increased cAMP levels. There were no changes in the voltage dependence or kinetics of Isk. Mutations that eliminate a potential phosphorylation site on the minK protein did not block the effects of activating the kinase. In addition, the membrane capacitance of the oocyte increased and decreased in parallel with Isk. Our results fit a mechanism in which channel proteins are selectively inserted into and removed from the plasma membrane in response to changes in kinase activity.


Published version. Journal of Neuroscience, Vol. 12, No. 1 (January 1992): 290-296. Permalink. © Society for Neuroscience 1992. Used with permission.

Edward Blumenthal was affiliated with the Yale University School of Medicine at the time of publication.

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