Document Type

Article

Language

eng

Format of Original

19 p.

Publication Date

10-1986

Publisher

Oxford University Press

Source Publication

Nucleic Acids Research

Source ISSN

0305-1048

Original Item ID

doi: 10.1093/nar/14.20.8007

Abstract

A small family of DNA sequences Is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which constitute the macronuclear family. Genomic Southern hybridization experiments indicate there are five members of the family in micronuclear DNA. All of the family members are present in whole genome homozygotes and are therefore nonallellic. The three macronuclear sequences are all present in clonal cell lines and are reproducibly generated in every developing macronucleus. The rearrangement event begins 14 hours after conjugation is initiated and is nearly completed by 16 hours.

Comments

Published version. Nucleic Acids Research, Vol. 14, No. 20 (October 1986): 8007-8025. DOI. © Oxford University Press 1986. Used with permission.

Kathleen Karrer was affiliated with Brandeis University at the time of publication.

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