Title

Nonrandom Localization of DNA Sequences in the Crescent Micronucleus of Tetrahymena

Document Type

Article

Language

eng

Format of Original

3 p.

Publication Date

8-1985

Publisher

International Society of Protistologists

Source Publication

The Journal of Protozoology

Source ISSN

0022-3921

Abstract

The premeiotic micronucleus of Tetrahymena thermophila elongates parallel to the long axis of the cell. In fixed cells one end of this crescent micronucleus appears thicker than the other, and either end may be oriented toward the anterior of the cell. Three families of repeated DNA sequences have been localized in the crescent micronucleus by in situ hybridization. Two micronucleus-specific sequences hybridize all along the crescent, but preferentially toward the ends. A macronucleus-retained sequence hybridizes preferentially to the half of the micronucleus at the thick end. Thus the arrangement of DNA sequences in the crescent micronucleus is nonrandom.

Comments

The Journal of Protozoology, Vol. 32, No. 3 (August 1985): 555-557. DOI.

Kathleen Karrer was affiliated with Brandeis University at the time of publication.