Title

T-DNA Transfer in Meristematic Cells of Maize Provided with Intracellular Agrobacterium

Document Type

Article

Language

eng

Format of Original

6 p.

Publication Date

8-1996

Publisher

Wiley

Source Publication

Plant Journal

Source ISSN

0960-7412

Abstract

Agrobacterium has been established as a tool for gene delivery to most dicotyledonous plant species. However, it is not generally efficient in monocotyledonous plant species, especially not in Graminae. In maize, Agrobacterium-mediated DNA transfer has been detected but early developmental stages in the plant proved incompetent as recipients. This research tests whether the lack of competence in young immature embryos of maize could be overcome by providing Agrobacterium in the interior of the plant cell. A microinjection technique was used to target single meristematic cells and prove competence to Agrobacterium. This response is dependent on the maize plant genotype.

Comments

Plant Journal, Vol. 10, No. 2 (August 1996): 355-360. DOI.

Michael Schläppi was affiliated with the Friedrich Miescher-Institut at the time of publication.