Document Type

Article

Language

eng

Format of Original

14 p.

Publication Date

3-2006

Publisher

Cold Spring Harbor Laboratory Press

Source Publication

RNA

Source ISSN

1355-8382

Original Item ID

doi: 10.1261/rna.2305406; PubMed Central, PMCID: PMC1383588

Abstract

1-Methyladenosine modification at position 58 of tRNA is catalyzed by a two-subunit methyltransferase composed of Trm6p and Trm61p in Saccharomyces cerevisiae. Initiator tRNA (tRNAiMet) lacking m1A58 (hypomethylated) is rendered unstable through the cooperative function of the poly(A) polymerases, Trf4p/Trf5p, and the nuclear exosome. We provide evidence that a catalytically active Trf4p poly(A) polymerase is required for polyadenylation of hypomethylated tRNAiMet in vivo. DNA sequence analysis of tRNAiMet cDNAs and Northern hybridizations of poly(A)+ RNA provide evidence that nascent pre-tRNAiMet transcripts are targeted for polyadenylation and degradation. We determined that a mutant U6 snRNA and an aberrant form of 5S rRNA are stabilized in the absence of Trf4p, supporting that Trf4p facilitated RNA surveillance is a global process that stretches beyond hypomethylated tRNAiMet. We conclude that an array of RNA polymerase III transcripts are targeted for Trf4p/ Trf5p-dependent polyadenylation and turnover to eliminate mutant and variant forms of normally stable RNAs.

Comments

Published version. RNA, Vol. 12, No. 3 (March 2006): 508-521. DOI. © Cold Spring Harbor Laboratory Press 2006. Used with permission.

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