Title

In vitro Uptake of Isotopically Labeled RNA by Mammalian Spleen Cells

Document Type

Article

Language

eng

Format of Original

6 p.

Publication Date

11-1965

Publisher

National Academy of Sciences

Source Publication

Proceedings of the Society for Experimental Biology and Medicine

Source ISSN

0037-9727

Abstract

Suspensions of rat spleen cells were found to incorporate P32 labeled rat spleen RNA. A maximal incorporation of 2.4% of the input RNA was obtained when 4 × 107 viable cells were cultured in the presence of 200 μg of P32 labeled RNA. Larger amounts of RNA appear to inhibit uptake. It was shown also that the uptake of P32 labeled RNA is competitively inhibited by unlabeled RNA, and that inhibition of uptake is proportional to the competitive RNA concentration. It was concluded that the majority of the radioactivity obtained from treated cells may be attributed to the uptake of RNA.

Comments

Proceedings of the Society for Experimental Biology and Medicine, Vol. 120, No. 2 (November 1965): 378-383. DOI.