Document Type

Article

Language

eng

Format of Original

12 p.

Publication Date

7-2011

Publisher

Wiley

Source Publication

Cytoskeleton

Source ISSN

1949-3584

Abstract

The unicellular alga Chlamydomonas can assemble two 10 μm flagella in 1 h from proteins synthesized in the cell body. Targeting and transporting these proteins to the flagella are simplified by preassembly of macromolecular complexes in the cell body. Radial spokes are flagellar complexes that are partially assembled in the cell body before entering the flagella. On the axoneme, radial spokes are “T” shaped structures with a head of five proteins and a stalk of 18 proteins that sediment together at 20S. In the cell body, radial spokes are partially assembled; about half of the radial spoke proteins (RSPs) form a 12S complex. In mutants lacking a single RSP, smaller spoke subassemblies were identified. When extracts from two such mutants were mixed in vitro the 12S complex was assembled from several smaller complexes demonstrating that portions of the stepwise assembly of radial spoke assembly can be carried out in vitro to elucidate the order of spoke assembly in the cell body.

Comments

Accepted version. Cytoskeleton, Vol. 68, No. 7 (July 2011): 389–400. DOI. © Wiley 2011. Used with permission.

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