Document Type

Article

Language

eng

Format of Original

6 p.

Publication Date

7-1988

Publisher

American Society for Microbiology

Source Publication

Journal of Bacteriology

Source ISSN

0021-9193

Original Item ID

PubMed Central, PMCID: PMC211263

Abstract

A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. The trpF, trpB, and trpA genes were on one cosmid, while the trpD and trpC genes were on two separate cosmids. The organization of the Z. mobilis trp genes seems to be similar to the organization found in Rhizobium spp., Acinetobacter calcoaceticus, and Pseudomonas acidovorans. The trpF, trpB, and trpA genes appeared to be linked, but they were not closely associated with trpD or trpC genes.

Comments

Published version. Journal of Bacteriology, Vol. 170, No. 7 (July 1988): 3158-3163. Permalink. © American Society for Microbiology 1988. Used with permission.

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