Document Type

Article

Language

eng

Format of Original

7 p.

Publication Date

4-2015

Publisher

Oxford University Press

Source Publication

FEMS Microbiology Letters

Source ISSN

0378-1097

Original Item ID

DOI: 10.1093/femsle/fnv029

Abstract

Among the few bacteria known to utilize methyl tert-butyl ether (MTBE) as a sole carbon source, Methylibium petroleiphilum PM1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its MTBE degradation pathway is limited. We investigated the role of a putative transcriptional activator gene, mdpC, in the induction of MTBE-degradation genes mdpA (encoding MTBE monooxygenase) and mdpJ (encoding tert-butyl alcohol hydroxylase) of strain PM1 in a gene-knockout mutant mdpC. We also utilized quantitative reverse transcriptase PCR assays targeting genes mdpA, mdpJ and mdpC to determine the effects of the mutation on transcription of these genes. Our results indicate that gene mdpC is involved in the induction of both mdpA and mdpJ in response to MTBE and tert-butyl alcohol (TBA) exposure in PM1. An additional independent mechanism may be involved in the induction of mdpJ in the presence of TBA.

Comments

Accepted version. FEMS Microbiology Letters, Vol. 362, No. 7 (April 2015). DOI. © FEMS 2015. Used with permission.

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