Title

A Comparison of Sarcoplasmic Reticulum Function in Fast and Slow Skeletal Muscle Using Crude Homogenate and Isolated Vesicles

Document Type

Article

Language

eng

Format of Original

7 p.

Publication Date

5-18-1981

Publisher

Elsevier

Source Publication

Life Sciences

Source ISSN

0024-3205

Abstract

Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca2+ uptake (Vmax and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca2+ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca2+ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.

Comments

Life Sciences, Vol. 28, No. 20 (May 1981): 2223-2229. DOI.