Title

Most, but not All, Yeast Strains in the Deletion Library Contain the [PIN+] Prion

Document Type

Article

Language

eng

Format of Original

8 p.

Publication Date

3-2010

Publisher

Wiley

Source Publication

Yeast

Source ISSN

0749-503X

Original Item ID

doi: 10.1002/yea.1740; PubMed Central, PMCID: PMC3717406

Abstract

The yeast deletion library is a collection of over 5100 single gene deletions that has been widely used by the yeast community. The presence of a non-Mendelian element, such as a prion, within this library could affect the outcome of many large-scale genomic studies. We previously showed that the deletion library parent strain contained the [PIN+] prion. [PIN+] is the misfolded infectious prion form of the Rnq1 protein that displays distinct fluorescent foci in the presence of RNQ1–GFP and exists in different physical conformations, called variants. Here, we show that over 97% of the library deletion strains are [PIN+]. Of the 141 remaining strains that have completely (58) or partially (83) lost [PIN+], 139 deletions were able to efficiently maintain three different [PIN+] variants despite extensive growth and storage at 4 °C. One strain, cue2Δ, displayed an alteration in the RNQ1–GFP fluorescent shape, but the Rnq1p prion aggregate shows no biochemical differences from the wild-type. Only strains containing a deletion of either HSP104 or RNQ1 are unable to maintain [PIN+], indicating that 5153 non-essential genes are not required for [PIN+] propagation. Copyright © 2009 John Wiley & Sons, Ltd.

Comments

Yeast, Vol. 27, No. 3 (March 2010): 159–166. DOI.