Document Type

Article

Language

eng

Format of Original

10 p.

Publication Date

7-2016

Publisher

Elsevier

Source Publication

Biochimica et Biophysica Acta: BBA - Molecular Cell Research

Source ISSN

0167-4889

Original Item ID

DOI: 10.1016/j.bbamcr.2016.04.012; PubMed PMID: 27091403

Abstract

Here we identified a hydrophobic 6.4 kDa protein, Cox26, as a novel component of yeast mitochondrial supercomplex comprising respiratory complexes III and IV. Multi-dimensional native and denaturing electrophoretic techniques were used to identify proteins interacting with Cox26. The majority of the Cox26 protein was found non-covalently bound to the complex IV moiety of the III–IV supercomplexes. A population of Cox26 was observed to exist in a disulfide bond partnership with the Cox2 subunit of complex IV. No pronounced growth phenotype for Cox26 deficiency was observed, indicating that Cox26 may not play a critical role in the COX enzymology, and we speculate that Cox26 may serve to regulate or support the Cox2 protein. Respiratory supercomplexes are assembled in the absence of the Cox26 protein, however their pattern slightly differs to the wild type III–IV supercomplex appearance. The catalytic activities of complexes III and IV were observed to be normal and respiration was comparable to wild type as long as cells were cultivated under normal growth conditions. Stress conditions, such as elevated temperatures resulted in mild decrease of respiration in non-fermentative media when the Cox26 protein was absent.

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta: BBA - Molecular Cell Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta: BBA - Molecular Cell Research, Vol. 1863, No. 7 pt. A (July 2016): 1643-1652. DOI. © Elsevier 2016. Used with permission.

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