Document Type

Conference Proceeding

Language

eng

Format of Original

11 p.

Publication Date

2-12-2000

Publisher

Society of Photo-Optical Instrumentation Engineers

Source Publication

Proceedings of SPIE 3978: Medical Imaging 2000: Physiology and Function from Multidimensional Images, San Diego, CA, (February 12, 2000)

Original Item ID

doi: 10.1117/12.383413

Abstract

We developed methods to quantify arterial structural and mechanical properties in excised rat lungs and applied them to investigate the distensibility decrease accompanying chronic hypoxia-induced pulmonary hypertension. Lungs of control and hypertensive (three weeks 11% O2) animals were excised and a contrast agent introduced before micro-CT imaging with a special purpose scanner. For each lung, four 3D image data sets were obtained, each at a different intra-arterial contrast agent pressure. Vessel segment diameters and lengths were measured at all levels in the arterial tree hierarchy, and these data used to generate features sensitive to distensibility changes. Results indicate that measurements obtained from 3D micro-CT images can be used to quantify vessel biomechanical properties in this rat model of pulmonary hypertension and that distensibility is reduced by exposure to chronic hypoxia. Mechanical properties can be assessed in a localized fashion and quantified in a spatially-resolved way or as a single parameter describing the tree as a whole. Micro-CT is a nondestructive way to rapidly assess structural and mechanical properties of arteries in small animal organs maintained in a physiological state. Quantitative features measured by this method may provide valuable insights into the mechanisms causing the elevated pressures in pulmonary hypertension of differing etiologies and should become increasingly valuable tools in the study of complex phenotypes in small-animal models of important diseases such as hypertension.

Comments

Published version. Proceedings of SPIE 3978: Medical Imaging 2000: Physiology and Function from Multidimensional Images, San Diego, CA, (February 12, 2000): 320-330. DOI. Used with permission.

Copyright 2000 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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