Title

Differentiation Between Rat Brain and Mouse Vas Deferens Delta Opioid Receptors

Document Type

Article

Language

eng

Format of Original

3 p.

Publication Date

2-1990

Publisher

Elsevier

Source Publication

European Journal of Pharmacology

Source ISSN

0014-2999

Original Item ID

doi: 10.1016/0014-2999(90)90556-L

Abstract

Certain enkephalin analogues, including those which contain the conformationally restricted amino acid E-(2R,3S)-cyclopropylphenylalanine ((2R,3S)-▿EPhe), have been shown to have high affinity for brain δ opioid receptors but are much less active in mouse vas deferens bioassays. To investigate whether there are differences between δ opioid receptors in brain and mouse was deferens, the ability of a selective δ opioid compound. [D-Pen2,pCl-Phe4,D-Pen5]enkephalin (pCl-DPDPE), and [D-Ala2,(2R,3S)-▿EPhe4,Leu5]enkephalin methyl ester (CP-OMe), to inhibit [3H]pCl-DPDPE binding in both rat brain and mouse vas deferens were measured. pCl-DPDPE recognized brain and mouse vas deferences binding sites with equal affinity, however, CP-OMe showed 33 fold lower affinity in mouse vas deferens compared to brain. This suggests that mouse vas deferens δ opioid receptors may be distinct from brain δ opioid receptors.

Comments

European Journal of Pharmacology, Vol. 177, No. 1-2 (February 1990): 99-101. DOI.