Differential Hydrogen Bonding in Human CYP17 Dictates Hydroxylation versus Lyase Chemistry

Authors

Michael Gregory, University of Illinois at Urbana-Champaign
Piotr J. Mak, Marquette UniversityFollow
Stephen G. Sligar, University of Illinois at Urbana-Champaign
James R. Kincaid, Marquette UniversityFollow

Document Type

Article

Publication Date

5-10-2013

Source Publication

Angewandte Chemie International Edition

Source ISSN

1433-7851

Abstract

Consequences of alternative H-bonding: Raman spectra of oxygenated intermediates of Nanodisc-incorporated human CYP17 in the presence of natural substrates (pregnenolone and progesterone) directly confirm that substrate structure effectively alters hydrogen-bonding interactions with the critical Fe–O–O fragment and dictates its predisposition for one of two alternative reaction pathways. Such substrate control has profound physiological implications.

Comments

Accepted version. Angewandte Chemie International Edition, Vol. 52, No. 20 (May 10, 2013): 5342-5345. DOI. © 2013 Wiley-VCH Verlag. Used with permission.

Recommended Citation

Gregory, Michael; Mak, Piotr J.; Sligar, Stephen G.; and Kincaid, James R., "Differential Hydrogen Bonding in Human CYP17 Dictates Hydroxylation versus Lyase Chemistry" (2013). Chemistry Faculty Research and Publications. 374.
https://epublications.marquette.edu/chem_fac/374