Some Considerations in Spectroelectrochemical Evaluation of Homogeneous Electron Transfer Involving Biological Molecules
Format of Original
American Chemical Society
Original Item ID
Potential-step chronoabsorptometry was used to measure the rate of the second-order homogeneous electron transfer between horse heart cytochrome c and methyl viologen radical. A rate constant of 1.0 X 106 ± 0.3M-1sec-1 was obtained at pH 7.2. Theoretical and experimental considerations affecting the measurement of rate constants are discussed. These include the deconvolution of time-dependent spectra and the influence of concentration and dlffusion coefficient ratios.