Electrochemical Studies of Cytochromes c

Authors

Michael D. Ryan, Marquette UniversityFollow
Benjamin A. Feinberg, University of Wisconsin-MilwaukeeFollow

Document Type

Article

Language

eng

Format of Original

5 p.

Publication Date

1978

Publisher

Elsevier

Source Publication

Bioelectrochemistry and Bioenergetics

Source ISSN

0302-4598

Abstract

Chronoamperometric methods were developed and used to study the reaction of iron-EDTA with horse cytochrome c and Rhodospirillum rubrum cytochrome c₂. These experiments served to demonstrate experimentally that for non-binding non-physiological redox agents, it is the overall protein charge that determines the kinetic-ionic strength relationships and not the localized charge of the presumed site of electron transfer. A theoretical discussion for earlier misconceptions of the simplified Debye-Hückel theory is presented. This new chronoamperometric approach to kinetics affords a relatively simple methodology for doing otherwise difficult kinetic studies under anaerobic conditions with easily autooxidizable redox agents such as ferrous-EDTA.

Comments

Bioelectrochemistry and Bioenergetics, Vol. 5, No. 3 (1978): 478-472. DOI.

Recommended Citation

Ryan, Michael D. and Feinberg, Benjamin A., "Electrochemical Studies of Cytochromes c" (1978). Chemistry Faculty Research and Publications. 501.
https://epublications.marquette.edu/chem_fac/501