Electrochemical Studies of Cytochromes c
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Bioelectrochemistry and Bioenergetics
Chronoamperometric methods were developed and used to study the reaction of iron-EDTA with horse cytochrome c and Rhodospirillum rubrum cytochrome c2. These experiments served to demonstrate experimentally that for non-binding non-physiological redox agents, it is the overall protein charge that determines the kinetic-ionic strength relationships and not the localized charge of the presumed site of electron transfer. A theoretical discussion for earlier misconceptions of the simplified Debye-Hückel theory is presented. This new chronoamperometric approach to kinetics affords a relatively simple methodology for doing otherwise difficult kinetic studies under anaerobic conditions with easily autooxidizable redox agents such as ferrous-EDTA.