Title

Analysis of Ultrafast Transient Absorption Spectroscopy Data using Integrative Data Analysis Platform: 1. Data Processing, Fitting, and Model Selection

Document Type

Article

Language

English

Publication Date

7-2017

Publisher

The Cold Spring Harbor Laboratory Press

Source Publication

bioRxiv beta: the Preprint Server for Biology

Abstract

This manuscript describes a workflow for analysis of transient absorption (TA) spectroscopy data using Integrative Data Analysis Platforms (IDAP) software package. Time-dependent spectral series are analyzed through evaluation of the isosbestic point and kinetics of excited state and ground-state bleach decays. Model fitting and selection based on Akaike's Information Criterion is discussed. As a practical example, we analyze excitation decays of a common protein label, Alexa Fluor 647.

Comments

bioRxiv beta: the Preprint Server for Biology (July 2017). DOI