Document Type
Article
Language
eng
Format of Original
5 p.
Publication Date
7-2013
Publisher
Oxford University Press
Source Publication
FEMS Microbiology Letters
Source ISSN
0378-1097
Original Item ID
doi: 10.1111/1574-6968.12153
Abstract
Triclosan is an antimicrobial agent that is discharged to soils with land-applied wastewater biosolids, is persistent under anaerobic conditions, and yet its impact on anaerobic microbial communities in soils is largely unknown. We hypothesized that triclosan enriches for Dehalococcoides-like Chloroflexi because these bacteria respire organochlorides and are likely less sensitive, relative to other bacteria, to the antimicrobial effects of triclosan. Triplicate anaerobic soil microcosms were seeded with agricultural soil, which was not previously exposed to triclosan, and were amended with 1 mg kg−1 of triclosan. Triplicate control microcosms did not receive triclosan, and the experiment was run for 618 days. The overall bacterial community (assessed by automated ribosomal intergenic spacer analysis and denaturing gradient gel electrophoresis) was not impacted by triclosan; however, the abundance of Dehalococcoides-like Chloroflexi 16S rRNA genes (determined by qPCR) increased 20-fold with triclosan amendment compared with a fivefold increase without triclosan. This work demonstrates that triclosan impacts anaerobic soil communities at environmentally relevant levels.
Recommended Citation
McNamara, Patrick J. and Krzmarzick, Mark J., "Triclosan Enriches for Dehalococcoides-like Chloroflexi in Anaerobic Soil at Environmentally Relevant Concentrations" (2013). Civil and Environmental Engineering Faculty Research and Publications. 3.
https://epublications.marquette.edu/civengin_fac/3
ADA Accessible Version
Comments
Accepted version. FEMS Microbiology Letters, Vol. 344, No. 1 (July 2013): 48-52. DOI. © 2013 Federation of European Microbiological Societies, published by John Wiley & Sons Ltd. Used with permission.