Title

Applicability of Quantitative PCR for Determination of Removal Efficacy of Enteric Viruses and Cryptosporidium by Water Treatment Processes

Document Type

Article

Language

eng

Format of Original

8 p.

Publication Date

2010

Publisher

IWA Publishing

Source Publication

Journal of Water and Health

Source ISSN

1477-8920

Original Item ID

doi: 10.2166/wh.2009.208

Abstract

This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.

Comments

Journal of Water and Health, Vol. 8, No. 1 (2010): 101-108. DOI.

Brooke Mayer was affiliated with the Arizona State University at the Tempe Campus at the time of publication.