Document Type

Article

Language

eng

Format of Original

4 p.

Publication Date

5-2016

Publisher

American Society for Microbiology

Source Publication

Journal of Clinical Microbiology

Source ISSN

0095-1137

Original Item ID

DOI: 10.1128/JCM.00271-16; PMCID: PMC4844743

Abstract

As an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. ProGastro SSC and Shiga toxin-producing Escherichia coli (i.e., STEC) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. Following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column methods. Mean processing times for manual and column methods were 30.5 and 69.2 min, respectively. Concordance of investigational methods with automated extraction was ≥98.8%.

Comments

Published version. Journal of Clinical Microbiology, Vol. 54, No. 5 (May 2016): 1384-1387. DOI. © American Society for Microbiology 2016. Used with permisison.

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