Document Type
Article
Language
eng
Format of Original
4 p.
Publication Date
5-2016
Publisher
American Society for Microbiology
Source Publication
Journal of Clinical Microbiology
Source ISSN
0095-1137
Original Item ID
DOI: 10.1128/JCM.00271-16; PMCID: PMC4844743
Abstract
As an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. ProGastro SSC and Shiga toxin-producing Escherichia coli (i.e., STEC) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. Following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column methods. Mean processing times for manual and column methods were 30.5 and 69.2 min, respectively. Concordance of investigational methods with automated extraction was ≥98.8%.
Recommended Citation
Munson, Erik; Napierala, Maureen; Munson, Kimber L.; Bilbo, Dorothy; and Schulte, Michael A., "Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens" (2016). Clinical Lab Sciences Faculty Research and Publications. 22.
https://epublications.marquette.edu/clinical_lab_fac/22
Comments
Published version. Journal of Clinical Microbiology, Vol. 54, No. 5 (May 2016): 1384-1387. DOI. © 2016 American Society for Microbiology. Used with permission.