Document Type

Article

Language

eng

Format of Original

6 p.; 24 cm

Publication Date

4-2016

Publisher

Elsevier

Source Publication

Acta Histochemica

Source ISSN

0065-1281

Original Item ID

doi: 10.1016/j.acthis.2016.02.006; PubMed Central, PMCID: PMC26947053

Abstract

The purpose of this study was to determine the presence of ADAM10 in temporomandibular joint disk with internal derangement. Twenty-five paraffin blocks of displaced temporomandibular joint (TMJ) disk specimens from earlier investigations were retrieved from the archives of the University of Catania. Of these 16 had been removed from females and 9 from males; 11 with anterior disk displacement with reduction (ADDwR) and 14 with anterior disk displacement without reduction (ADDwoR). The sections were dehydrated, embedded in paraffin and cut. Then they were incubated in 0.3% H2O2/methanol and half of sections from each sample were incubated in diluted rabbit polyclonal anti-ADAM10 antibody. Then biotinylated anti-mouse/anti-rabbit IgG was applied to the sections, followed by avidin–biotin–perioxidase complex. The results were analyzed and the results were that ADAM10 was overexpressed in the posterior band of sections from patients with ADDwR compared to the other bands of both ADDwR and ADDwoR sections. Overexpression correlated with severe histopathological degeneration. We believe these results have the potential to provide insights into the pathogenesis of TMJ disk degeneration and to help design new therapeutic approaches targeting the proteolytic events that lead to tissue degeneration. Early therapeutic block of ADAM10 activity could succeed in limiting aggrecan-rich matrix breakdown without affecting normal physiology.

Comments

Accepted version. Acta Histochemica, Vol. 118, No. 3 (April 2016): 293-298. DOI. © Elsevier 2016. Used with permission.

NOTICE: this is the author’s version of a work that was accepted for publication in Acta Histochemica. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Histochemica, VOL. 118, ISSUE 3, April 2016, DOI.

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