Effect of Photobiomodulation on Vinblastine-Poisoned Murine HERS Cells

Authors

Brian D. Hodgson, Marquette UniversityFollow
B. Pyszka
Michele M. Henry, Medical College of Wisconsin
E. Buchmann, Medical College of WisconsinFollow
Harry T. Whelan

Document Type

Article

Language

eng

Format of Original

5 p.

Publication Date

2010

Publisher

Mary Ann Liebert Inc.

Source Publication

Photomedicine and Laser Surgery

Source ISSN

1549-5419

Original Item ID

doi: 10.1089/pho.2010.2794

Abstract

Objective: The aim of this study was to investigate the effect of near-infrared (NIR) photobiomodulation on the proliferation and glutathione levels in murine Hertwig's epithelial root sheath (HERS) cells after poisoning with vinblastine. Background: Photobiomodulation has been shown to improve wound healing in a number of animal models. There have been no studies on the effect of photobiomodulation on cancer-related chemotherapy injury to the cells that initiate tooth root growth. Materials and Methods: Control groups consisted of murine HERS cells without vinblastine (VB−) and cells with vinblastine at 10, 20, and 30 ng/mL (VB10, VB20, and VB30). Experimental groups consisted of these same groups with light therapy (VB-L, VB10L, VB20L, and VB30L). The cells were exposed to vinblastine for 1 h. Photobiomodulation consisted of a 75-cm² gallium-aluminum-arsenide light-emitting diode (LED) array at an energy density of 12.8 J/cm², delivered with 50 mW/cm² power over 256 s. Results: Vinblastine alone significantly decreased HERS cell proliferation and glutathione levels at all concentrations (VB10 [−55%, p < 1.0 × 10⁻⁸]; VB20 [−72%, p < 1.0 × 10⁻⁹]; VB30 [−80%, p < 1.0 × 10⁻¹⁰]; and VB10 [−36%, p < 0.0001]; VB20 [−49%, p < 1.0 × 10⁻⁶]; VB30 [−53%, p < 1.0 × 10⁻⁷] respectively). Photobiomodulation significantly increased cell proliferation at all levels of vinblastine exposure (VB10L [+50%, p < 0.0001]; VB20L [+45%, p < 0.05]; VB30 [+39%, p < 0.05]) but not of the control (+22%, p  = 0.063). The photobiomodulation significantly increased glutathione production in all concentrations of vinblastine except 20 ng/mL (VB10L [+39%, p = 0.007]; VB20L [+19%, p = 0.087]; VB30 [+14%, p = 0.025]) and the control (+12%, p = 0.13). Conclusions: Photobiomodulation demonstrated an improvement in proliferation and glutathione levels in vinblastine-poisoned murine HERS cells.

Comments

Published version. Photomedicine and Laser Surgery, Volume 29, No. 4 (2011): 233–237. DOI. © 2011 Mary Ann Liebert Inc. Used with permission.

Recommended Citation

Hodgson, Brian D.; Pyszka, B.; Henry, Michele M.; Buchmann, E.; and Whelan, Harry T., "Effect of Photobiomodulation on Vinblastine-Poisoned Murine HERS Cells" (2010). School of Dentistry Faculty Research and Publications. 21.
https://epublications.marquette.edu/dentistry_fac/21