Title

In-vivo Single Cell Protein Interaction Investigation using Microfluidic Platform

Document Type

Conference Proceeding

Language

eng

Format of Original

4 p.

Publication Date

6-21-2015

Publisher

Institute of Electrical and Electronics Engineers (IEEE)

Source Publication

2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

Source ISSN

2159-547X

Abstract

We have developed a microfluidic platform to immobilize single living cells at a position in a microfluidic channel for monitoring cell responses to different stimuli over time. While the cell is trapped, the device maintains control over changing the channel media and flow rate. The captured cell may be released in order to capture another cell and repeat the experiment without disturbing the setup. We have demonstrated the monitoring of an individual yeast cell, in which the captured cell is exposed to α-factor ligand.

Comments

Published as part of the proceedings of the 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015: 1541-1544. DOI.