Document Type

Article

Language

eng

Format of Original

8 p.

Publication Date

10-2004

Publisher

American Society for Microbiology

Source Publication

Infection and Immunity

Source ISSN

0019-9567

Original Item ID

doi: 10.1128/IAI.72.10.6087-6094.2004; PubMed Central: PMCID 517601

Abstract

Allergic aspergillosis is a Th2 T-lymphocyte-mediated pulmonary complication in patients with atopic asthma and cystic fibrosis. Therefore, any therapeutic strategy that selectively inhibits Th2 T-cell activation may be useful in downregulating allergic lung inflammation in asthma. In the present study, we developed a CpG oligodeoxynucleotide (ODN)-based immune intervention of allergic inflammation in a mouse model of allergic aspergillosis. Four different groups of mice were used in a short-term immunization protocol. Three experimental groups of animals (groups 1 to 3) were sensitized with Aspergillus fumigatus antigens. Animals in group 1 were immunized with A. fumigatus antigen alone, while those in group 2 were treated with CpG–ODN 1 day before the first antigen immunization, and the animals in group 3 received the first CpG–ODN administration between the antigen treatments. The animals in group 4 served as controls and were given phosphate-buffered saline. Allergen-specific serum immunoglobulins and total immunoglobulin E in different groups of animals were measured by enzyme-linked immunosorbent assay, while airway remodeling and cytokine production were studied by immunohistochemistry. The results demonstrated that CpG–ODN administration either before (group 2) or between (group 3) antigen treatments resulted in reduced total immunoglobulin E levels and peripheral blood eosinophil numbers compared to A. fumigatus allergen-sensitized group 1 animals. Similarly, treatment with CpG–ODN also downregulated inflammatory cell infiltration, goblet cell hyperplasia, and basement membrane thickening compared to A. fumigatus- sensitized mice. The distinct reduction in peripheral blood eosinophilia and airway remodeling in CpG–ODN-treated mice emphasized its usefulness as an immunomodulating agent or allergic fungal diseases.

Comments

Published version. Infection and Immunity, Vol. 72, No. 10 (October 2004): 6087-6094. DOI. © American Society for Microbiology 2004. Used with permission.

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