Document Type

Article

Language

eng

Format of Original

9 p.

Publication Date

2009

Publisher

BioMed Central

Source Publication

BMC Bioinformatics

Source ISSN

1471-2105

Original Item ID

doi: 10.1186/1471-2105-10-217

Abstract

Background: Physical maps have been historically one of the cornerstones of genome sequencing and map-based cloning strategies. They also support marker assisted breeding and EST mapping. The problem of building a high quality physical map is computationally challenging due to unavoidable noise in the input fingerprint data. Results: We propose a novel compartmentalized method for the assembly of high quality physical maps from fingerprinted clones. The knowledge of genetic markers enables us to group clones into clusters so that clones in the same cluster are more likely to overlap. For each cluster of clones, a local physical map is first constructed using FingerPrinted Contigs (FPC). Then, all the individual maps are carefully merged into the final physical map. Experimental results on the genomes of rice and barley demonstrate that the compartmentalized assembly produces significantly more accurate maps, and that it can detect and isolate clones that would induce "chimeric" contigs if used in the final assembly. Conclusion: The software is available for download at http://www.cs.ucr.edu/~sbozdag/ assembler/

Comments

Published version. BMC Bioinformatics, Vol. 10 (2009): 217-229. DOI. © 2009 BioMed Central. Used with permission.


This work is licensed under a Creative Commons Attribution 3.0 Unported License.

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