Title

Identification of Periplasmic Nitrate Reductase Mo(V) EPR Signals in Intact Cells of Paracoccus denitrificans

Document Type

Article

Language

eng

Format of Original

4 p.

Publication Date

8-15-1995

Publisher

Portland Press Limited

Source Publication

Biochemical Journal

Source ISSN

0264-6021

Abstract

EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.

Comments

Biochemical Journal, Vol. 310, No. 1 (August 15, 1995): 311-314. Permalink.

Brian Bennett was affiliated with the University of East Anglia at the time of publication.