Document Type

Article

Language

eng

Format of Original

13 p.

Publication Date

5-2009

Publisher

Springer

Source Publication

Journal of Biological Inorganic Chemistry

Source ISSN

0949-8257

Original Item ID

doi: 10.1007/s00775-009-0471-2; PubMed Central: PMCID 2678238

Abstract

Methionine aminopeptidases (MetAPs) represent a unique class of protease that is capable of the hydrolytic removal of an N-terminal methionine residue from nascent polypeptide chains. MetAPs are physiologically important enzymes; hence, there is considerable interest in developing inhibitors that can be used as antiangiogenic and antimicrobial agents. A detailed kinetic and spectroscopic study has been performed to probe the binding of a triazole-based inhibitor and a bestatin-based inhibitor to both Mn(II)- and Co(II)-loaded type-I (Escherichia coli) and type-II (Pyrococcus furiosus) MetAPs. Both inhibitors were found to be moderate competitive inhibitors. The triazole-type inhibitor was found to interact with both active-site metal ions, while the bestatin-type inhibitor was capable of switching its mode of binding depending on the metal in the active site and the type of MetAP enzyme.

Comments

Accepted version. Journal of Biological Inorganic Chemistry, Vol.14, No. 4 (May 2009): 573-585. DOI. © 2009 Springer. Used with permission.

The final publication is available at Springer via http://dx.doi.org/10.1007/s00775-009-0471-2.

Brian Bennett was affiliated with Medical College of Wisconsin at the time of publication.

Richard Holz was affiliated with Loyola University-Chicago at the time of publication.

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Physics Commons

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