Date of Award

Summer 1990

Degree Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Biology

Abstract

The major prolactin (PRL)-inducible gene product in the ptgeon cropsac (cp35) is a member of the lipocortin/calpactin family. In an attempt to study the molecular mechanisms of the action of PRL in the pigeon model, I have examined the distribution of the mRNAcp35 by Northern analysis of RNA from various pigeon tissues. In addition, I have studied the role of protein synthesis during the induction of cropsac mRNAcp35 by PRL by measuring the level of mRNAcp35 from the cropsac under the influence of PRL and cycloheximide (CHX). Among eight different organs in addition to cropsac dissected from pigeons, trachea, lung, spleen and intestine expressed moderate levels of mRNA which hybridize to cp35 eDNA. Heart and skeletal muscle did not express detectable cp35 mRNA. PRL had no effect on the expression of the cp35-related mRNAs in any of these tissues. This result indicates that the regulation of cp35 gene is a unique event of the cropsac response to PRL. Pigeons responded with a significant induction of cp35 mRNA m the cropsac when injected with PRL and CHX in different temporal relationships, indicating that the induction may not require ongoing protein synthesis. I am very grateful to all my teachers and friends. Without their help, it is impossible for me to have such a wonderful chance of studying and continuing this line of work.

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