Date of Award

Spring 1978

Degree Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Biology

Abstract

Neurospora crassa grows on medium and long chain fatty acids but not on short chain fatty acids. Enzymatic assays of oleate grown cells indicated that acyl-CoA synthetase, 3-hydroxyl-CoA dehydrogenase, crotonase and thiolase were induced. The activity of acyl-CoA dehydrogenase in glucose, acetate or oleate grown cells indicated that it is not induced, and thus not co-ordinately controlled with the other fad enzymes. All these enzymes, with the exception of the thiolase was found predominantly in the mitochondrial fraction. Most of the thiolase activity was found in the cytoplasm. Six mutants with fad- phenotype as characterized by the lack of growth on oleate were isolated. In addition three partial mutants with reduced growth on oleate were isolated. The enzymes of the partial, and fad- mutants showed that they had reduced fad- enzyme levels. Crosses of mutants to wild type, 74-A, and "Alcoy" were performed to map the mutation. Complementation studies indicated that there were at least seven complementing groups.

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