Date of Award

Spring 2016

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Dentistry

First Advisor

Stover, Sheila

Second Advisor

Tayebi, Lobat

Third Advisor

Hashimoto, Lance

Abstract

Introduction: Viability of periodontal ligament (PDL) cells is a key prognostic factor fora replanted tooth following avulsion. Dehydration results death of PDL cells, which inturn causes ankylosis upon replantation, followed by replacement resorption.Hank’s Balanced Salt Solution (HBSS) has been recommended as the standardstorage medium for an avulsed tooth. Save-A-Tooth and EMT Toothsaver (Dentosafe)are commercially available tooth storage media. With the best of our knowledge no studyhas yet compared these media for their effective tooth storage times. The aim of this invitro study is to evaluate Save-A-Tooth, EMT Toothsaver (Dentosafe), and HBSS fortheir effect on the viability of human periodontal ligament fibroblast (HPDLF).Methods: Cultured primary HPDLFs were seeded on 96-well cell culture plates andexposed to water (negative control), HBSS, Save-A-Tooth and EMT Toothsaver(Dentosafe) at room temperature (22°C) for 0.5, 1, 3, 6, 12, 24 or 48 hours. After eachexposure time, cell viability was measured by quantifying ATP present using aluminescent dye. The data was statistically analyzed by ANOVA and post hoc LeastSignificant Difference (LSD) test (P < 0.05).Results: There was no difference in cell viability among HBSS, Save-A-Tooth and EMTToothsaver (Dentosafe) groups for up to 6 hours. Save-A-Tooth was effective only up to12 hours and then became detrimental to HPDLF; at 24 and 48 hours, the effectiveness ofSave-A-Tooth was similar to that of water. Starting from the 24-hour time-point, EMTToothsaver (Dentosafe) was more effective than any other tested media. The number ofviable cells exposed to EMT Toothsaver (Dentosafe) and HBSS increased between the24- and 48-hour time-points.Conclusions: EMT Toothsaver (Dentosafe) is the best tooth storage medium among thetested media, although HBSS was similarly effective. Save-A-Tooth is not suitable tostore an avulsed tooth for greater than 6 hours.

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