Date of Award

2-1944

Degree Type

Bachelors Essay

Degree Name

Bachelor of Science (BS)

Department

Biological Sciences

First Advisor

William N. Steil

Second Advisor

M.S. Barnett

Abstract

Dehydration is the process of removing water from tissue which has been fixed and hardened. It is a necessary step for the Infiltration of paraffin. If the dehydrating agent is not a solvent of the paraffin, it is necessary to use a solvent first and then infiltrate with paraffin. Dehydration, according to Sass (1940), has some washing action besides making the material firm and, in some cases, hard and brittle. When Bouin’s fixing fluid is used, ethyl alcohol is the solvent used in removing the solution and in washing the material. Dehydrating agents which possess hygrosopic properties are those most commonly used for dehydrating tissues. The best dehydrating fluid, according to Johansen (1935), is one which mixes equally well with water, ethyl alcohol, balsam and paraffin, and which does not produce desiccation of the tissue.

Comments

A thesis presented to the Faculty of the College of Liberal Arts of the Marquette University in partial fulfillment of the Requirements for the Degree of Bachelor of Science, Milwaukee, Wisconsin

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