Radial Spoke Proteins of Chlamydomonas Flagella

Authors

Pinfen Yang, Marquette UniversityFollow
Dennis R. Diener, Yale University
Chun Yang, Marquette UniversityFollow
Takahiro Kohno, University of Tokyo
Gregory J. Pazour, University of Massachusetts Medical School WorcesterFollow
Jennifer M. Dienes, Marquette University
Nathan S. Agrin, University of Massachusetts Medical School Worcester
Stephen M. King, University of Connecticut Health CenterFollow
Winfield S. Sale, Emory University School of MedicineFollow
Ritsu Kamiya, University of Tokyo
Joel L. Rosenbaum, Yale University
George B. Witman, University of Massachusetts Medical School Worcester

Document Type

Article

Language

eng

Format of Original

10 p.

Publication Date

3-2006

Publisher

Company of Biologists

Source Publication

Journal of Cell Science

Source ISSN

0021-9533

Abstract

The radial spoke is a ubiquitous component of `9+2' cilia and flagella, and plays an essential role in the control of dynein arm activity by relaying signals from the central pair of microtubules to the arms. The Chlamydomonas reinhardtii radial spoke contains at least 23 proteins, only 8 of which have been characterized at the molecular level. Here, we use mass spectrometry to identify 10 additional radial spoke proteins. Many of the newly identified proteins in the spoke stalk are predicted to contain domains associated with signal transduction, including Ca²⁺-, AKAP- and nucleotide-binding domains. This suggests that the spoke stalk is both a scaffold for signaling molecules and itself a transducer of signals. Moreover, in addition to the recently described HSP40 family member, a second spoke stalk protein is predicted to be a molecular chaperone, implying that there is a sophisticated mechanism for the assembly of this large complex. Among the 18 spoke proteins identified to date, at least 12 have apparent homologs in humans, indicating that the radial spoke has been conserved throughout evolution. The human genes encoding these proteins are candidates for causing primary ciliary dyskinesia, a severe inherited disease involving missing or defective axonemal structures, including the radial spokes.

Comments

Accepted version. Journal of Cell Science, Vol. 119, Pt. 6 (March 2006): 1165-1174. DOI. © 2006 Company of Biologists. Used with permission.

Recommended Citation

Yang, Pinfen; Diener, Dennis R.; Yang, Chun; Kohno, Takahiro; Pazour, Gregory J.; Dienes, Jennifer M.; Agrin, Nathan S.; King, Stephen M.; Sale, Winfield S.; Kamiya, Ritsu; Rosenbaum, Joel L.; and Witman, George B., "Radial Spoke Proteins of Chlamydomonas Flagella" (2006). Biological Sciences Faculty Research and Publications. 324.
https://epublications.marquette.edu/bio_fac/324