Studies of the Chicken Immune Response. III. Cellular and Humoral Antibody Production in the Splenectomized Chicken
Format of Original
American Association of Immunologists
The Journal of Immunology
Young chickens, when bleeding or rupture of the spleen accompanied splenectomy, often regenerated small spleens (splenules) in 4 weeks. These splenules, at 8 weeks, were found to be capable of producing antibody, as tested by the Jerne plaque technique. The bone marrow of such chickens was also functional, although to a less extent. When splenectomy was carried out with no bleeding or rupture, no splenules occurred. In these chickens, the introduction of sheep red blood cells 4 weeks later led to the occurrence of antibody-producing cells in the bone marrow but not in the bursa, thymus, liver, lung or cecal lymph. The serum hemagglutinin titer was always lower in splenectomized chickens than in controls, and always rose more slowely and reached its peak titer later. The IgM fraction appeared sooner than the IgG fraction, but the ratio of the two components for 7 days was similar in all groups. There was no significant difference in time of rejection of skin homografts in control and splenectomized chickens.
Keily, Sister Danile and Abramoff, Peter, "Studies of the Chicken Immune Response. III. Cellular and Humoral Antibody Production in the Splenectomized Chicken" (1969). Biological Sciences Faculty Research and Publications. 391.