Format of Original
American Society for Microbiology
Journal of Bacteriology
Original Item ID
PubMed Central, PMCID: PMC251555
A temperature-sensitive mutant of Escherichia coli in which the synthesis of l-arabinose isomerase is blocked during growth at 42 C was found to possess the following properties. (i) The mutation occurred in the structural gene for the isomerase, gene araA. (ii) During growth at elevated temperatures the mutant accumulates a product which is a precursor to the active enzyme. (iii) The precursor produced at 42 C is slowly converted to active enzyme at 28 C in the absence of protein and ribonucleic acid synthesis. It is concluded that the mutation results in a change in the structure of isomerase which causes formation of active enzyme to be thermolabile at a step beyond the level of translation.
Pauley, Judith; Power, Joseph; and Irr, Joseph, "L-Arabinose Isomerase Formation in a Conditional Mutant of Gene araA of Escherichia coli B/r" (1972). Biological Sciences Faculty Research and Publications. 401.