Inactivation of Yeast Hexokinase B by Triethyltin Bromide
American Chemical Society
Original Item ID
Triethyltin bromide was found to demonstrate temperature-dependent inactivation of yeast hexokinase B. At temperatures of 20 °C or lower, little or no inactivation of the enzyme was detected after 2 h of reaction with 50-300 µ concentrations of the reagent. However, incubation at 25 °C or higher resulted in an increased rate and extent of loss of the enzyme activity with increasing incubation temperatures. The Arrhenius plot for the inactivation process showed a sharp break at approximately 30 °C, with a heat of activation (∆H*) above this temperature of 55.2 kcal, indicating that a triethyltin-induced conformational change occurred at the elevated temperatures. Sugar substrates provided protection
Siebenlist, Kevin R. and Taketa, Fumito, "Inactivation of Yeast Hexokinase B by Triethyltin Bromide" (1983). Biomedical Sciences Faculty Research and Publications. 225.