Title

Inactivation of Yeast Hexokinase B by Triethyltin Bromide

Document Type

Article

Publication Date

8-1-1983

Publisher

American Chemical Society

Source Publication

Biochemistry

Source ISSN

0006-2960

Original Item ID

DOI: 10.1021/bi00287a011

Abstract

Triethyltin bromide was found to demonstrate temperature-dependent inactivation of yeast hexokinase B. At temperatures of 20 °C or lower, little or no inactivation of the enzyme was detected after 2 h of reaction with 50-300 µ concentrations of the reagent. However, incubation at 25 °C or higher resulted in an increased rate and extent of loss of the enzyme activity with increasing incubation temperatures. The Arrhenius plot for the inactivation process showed a sharp break at approximately 30 °C, with a heat of activation (∆H*) above this temperature of 55.2 kcal, indicating that a triethyltin-induced conformational change occurred at the elevated temperatures. Sugar substrates provided protection

Comments

Biochemistry, Vol. 22, No. 18 (August 1, 1983): 4229-4234. DOI.

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