Electrochemical Studies of Cytochromes c
Format of Original
Bioelectrochemistry and Bioenergetics
Chronoamperometric methods were developed and used to study the reaction of iron-EDTA with horse cytochrome c and Rhodospirillum rubrum cytochrome c2. These experiments served to demonstrate experimentally that for non-binding non-physiological redox agents, it is the overall protein charge that determines the kinetic-ionic strength relationships and not the localized charge of the presumed site of electron transfer. A theoretical discussion for earlier misconceptions of the simplified Debye-Hückel theory is presented. This new chronoamperometric approach to kinetics affords a relatively simple methodology for doing otherwise difficult kinetic studies under anaerobic conditions with easily autooxidizable redox agents such as ferrous-EDTA.
Ryan, Michael D. and Feinberg, Benjamin A., "Electrochemical Studies of Cytochromes c" (1978). Chemistry Faculty Research and Publications. 501.