Date of Award
Thesis - Restricted
Master of Science (MS)
Mathematics, Statistics and Computer Science
Tonellato, Peter J.
Clough, Anne V.
Jacob, Howard J.
Expressed Sequence Tags (EST) are short DNA sequences derived from single pass sequencing of reverse transcribed RNA libraries. Together with other gene-derived sequences, ESTs are computationally clustered as UniGenes to represent the theoretical predictions of biological genes. The accuracy of gene homology analysis based on UniGene sequence similarity directly affects both the computational and wet lab methods that rely on the UniGenes and predicted homologs. In this study, the optimal DNA sequence alignment criteria for identification of orthologous UniGenes between rat and mouse and between mouse and human were systematically analyzed and determined. Furthermore, scoring algorithms designed to extract unique orthologous UniGene pairs from complex UniGene "objects" were implemented and tested in conjunction with the DNA sequence alignment criteria. The results from this study have been successfully applied to comparative gene mapping and analysis.
Chen, Yongjian Samuel, "Computational Analysis of Expressed Sequence Tags and Unigenes" (2000). Master's Theses (1922-2009) Access restricted to Marquette Campus. 2118.