Date of Award

Spring 1962

Degree Type

Thesis - Restricted

Degree Name

Master of Science (MS)



First Advisor

Laskowski, Michael

Second Advisor

Peanasky, Robert J.

Third Advisor

Haymaker, C. R.


Various leguminosae have been found to contain compounds which inhibit the proteolytic action of trypsin. In 1949, Tauber et al. (30) reported that protein crystals obtained from lima beans had the power to inhibit the proteolytic action of trypsin. A few years later, however, Fraenkel-Conrat et al. (6) by modifying Tauber's recrystallization method found that the mother liquor contained a high trypsin inhibiting activity. The crystals were found to be devoid of activity after two recrystallizations. Fraenkel-Conrat's preparation was two and one half times as powerful as Tauber's inhibitor. Recently, Jirgensons et al. (12) found that Fraenkel-Conrat's preparation can be further subdivided by DEAE-cellulose column chromatography into at least six fractions, five of which possessed almost identical activities. This thesis was undertaken to attempt the use of chromatography as a simpler method of separating the active lima bean trypsin inhibitor from Tauber's inactive protein crystals and to define conditions for separation of fractions observed by Jirgensons.