Date of Award
Summer 2011
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biomedical Engineering
First Advisor
Carroll, Joseph
Second Advisor
Gilat-Schmidt, Taly
Third Advisor
Ropella, Kristina
Abstract
Optical Coherence Tomography (OCT) has allowed in-vivo viewing of details of retinal layers like never before. With the development of spectral domain OCT (SD-OCT) details of nearly 2µm axial resolution and higher imaging speed have been reported. Nevertheless, a single volume scan of the retina is typically restricted to 6mm x 6mm in size. Having a larger field of view of the retina will definitely enhance the clinical utility of the OCT.
A tool was developed for creating wide-field thickness maps of the retina by combining the use of already available tools like i2k Retina (DualAlign, LLC, Clifton Park, NY) and the thickness maps from Cirrus HD-OCT research browser (Carl Zeiss Meditec, Dublin, California, USA). Normal subjects (n=20) were imaged on Zeiss Cirrus HD-OCT using 512x128 Macular Cube scanning protocol. Sixteen overlapping volumetric images were obtained by moving the internal fixation target around such that the final stitched maps were 12mm x 14mm in size. The thickness maps were corrected for inter-individual differences in axial lengths measured using Zeiss IOL Master and averaged to obtain a normative map.
An algorithm was also developed for montaging 3-D volume scans. Using this algorithm two OCT volume scans can be registered and stitched together to obtain a larger volume scan. The algorithm can be described as a two step process involving 3-D phase-correlation and 2-D Pseudo-polar Fourier transform (PPFT). In the first step, 3-D phase-correlation provides translation values in the x, y and z axis. The second step involves applying PPFT on each overlapping pair of B-scans to find rotation in the x-y plane. Subsequent volumes can be stitched to obtain a large field of view.
We developed a simple and robust method for creating wide-field views of the retina using existing SD-OCT hardware. As segmentation algorithms improve, this method could be expanded to produce wide-field maps of retinal sub-layers, such as the outer nuclear layer or retinal nerve fiber layer. These wide-field views of the retina may prove useful in evaluating retinal diseases involving the peripheral retina (e.g., retinitis pigmentosa and glaucoma).