Water Transport and the Distribution of Aquaporin-1 in Pulmonary Air Spaces

Authors

Richard M. Effros, Medical College of Wisconsin
C. Darin, Medical College of Wisconsin
Elizabeth R. Jacobs, Medical College of Wisconsin
R. A. Rogers, Harvard University
Gary S. Krenz, Marquette UniversityFollow
E. E. Schneeberger, Massachusetts General Hospital

Document Type

Article

Language

eng

Format of Original

15 p.

Publication Date

9-1997

Publisher

American Physiological Society

Source Publication

Journal of Applied Physiology

Source ISSN

0021-8987

Original Item ID

DOI: 10.1152/jappl.1997.83.3.1002

Abstract

Recent evidence suggests that water transport between the pulmonary vasculature and air spaces can be inhibited by HgCl₂, an agent that inhibits water channels (aquaporin-1 and -5) of cell membranes. In the present study of isolated rat lungs, clearances of labeled (³HOH) and unlabeled water were compared after instillation of hypotonic or hypertonic solutions into the air spaces or injection of a hypotonic bolus into the pulmonary artery. The clearance of ³HOH between the air spaces and perfusate after intratracheal instillation and from the vasculature to the tissues after pulmonary arterial injections was invariably greater than that of unlabeled water, indicating that osmotically driven transport of water is limited by permeability of the tissue barriers rather than the rate of perfusion. Exposure to 0.5 mM HgCl₂ in the perfusate and air-space solution reduced the product of the filtration coefficient and surface area (P _f S) of water from the air spaces to the perfusate by 28% after instillation of water into the trachea. In contrast, perfusion of 0.5 mM HgCl₂ in air-filled lungs reducedP _f Sof the endothelium by 86% after injections into the pulmonary artery, suggesting that much of the action of this inhibitor is on the endothelial surfaces. Confocal laser scanning microscopy demonstrated that aquaporin-1 is on mouse pulmonary endothelium. No aquaporin-1 was found on alveolar type I cells with immunogold transmission electron microscopy, but small amounts were present on some type II cells.

Comments

Accepted version. Journal of Applied Physiology, Vol. 83, No. 3, (September 1997): 1002-1016. DOI. © 1997 American Physiological Society. Used with permission.

Recommended Citation

Effros, Richard M.; Darin, C.; Jacobs, Elizabeth R.; Rogers, R. A.; Krenz, Gary S.; and Schneeberger, E. E., "Water Transport and the Distribution of Aquaporin-1 in Pulmonary Air Spaces" (1997). Biomedical Engineering Faculty Research and Publications. 15.
https://epublications.marquette.edu/bioengin_fac/15