Mechanism of Preconditioning by Isoflurane in Rabbits: A Direct Role for Reactive Oxygen Species

Authors

Katsuya Tanaka, Medical College of WisconsinFollow
Dorothee Weihrauch, Medical College of Wisconsin
Franz Kehl, Medical College of Wisconsin
Lynda M. Ludwig, Medical College of Wisconsin
John F. LaDisa, Marquette UniversityFollow
Judy R. Kersten, Medical College of Wisconsin
Paul S. Pagel, Medical College of WisconsinFollow
David C. Warltier, Medical College of WisconsinFollow

Document Type

Article

Language

eng

Format of Original

6 p.

Publication Date

12-2002

Publisher

Lippincott Williams & Wilkins, Inc.

Source Publication

Anesthesiology

Source ISSN

0003-3022

Abstract

LARGE quantities of reactive oxygen species (ROS) released during reperfusion after coronary artery occlusion damage proteins responsible for intracellular homeostasis, produce tissue injury, depress contractile function, and increase myocardial infarct size. In contrast, small quantities of ROS may exert beneficial effects during ischemia and reperfusion when released before a prolonged ischemic event. ROS derived from mitochondria during a brief ischemic episode produce preconditioning. Free radical scavengers administered during ischemic preconditioning (IPC) markedly attenuate the protective effect of the preconditioning stimulus on infarct size. These data suggest that IPC is mediated in part by small quantities of ROS released during preconditioning. Volatile anesthetics protect myocardium against infarction through a signal transduction pathway that includes adenosine type 1 receptors, protein kinase C, inhibitory guanine regulatory proteins, and mitochondrial and sarcolemmal adenosine triphosphate-regulated potassium (K^ATP) channels. A recent investigation by Müllenheim et al . provides compelling evidence that ROS also mediate myocardial protection produced by volatile anesthetics. We sought to confirm and extend these important results by examining the hypothesis that ROS scavengers inhibit isoflurane-induced protection against irreversible ischemic injury. We further tested the hypothesis that isoflurane directly generates ROS in rabbit ventricular myocardium in vivo using a confocal microscopic technique combined with the superoxide anion-specific fluorescent probe dihydroethidium.

Comments

Accepted version. Anesthesiology, Vol. 97, No. 6 (December 2002): 1485-1490. DOI. © 2002 American Society of Anesthesiologists. Used with permission.

John LaDisa was affiliated with the Medical College of Wisconsin at the time of publication.

Recommended Citation

Tanaka, Katsuya; Weihrauch, Dorothee; Kehl, Franz; Ludwig, Lynda M.; LaDisa, John F.; Kersten, Judy R.; Pagel, Paul S.; and Warltier, David C., "Mechanism of Preconditioning by Isoflurane in Rabbits: A Direct Role for Reactive Oxygen Species" (2002). Biomedical Engineering Faculty Research and Publications. 224.
https://epublications.marquette.edu/bioengin_fac/224