A Defined Agar Medium for Genetic Transformation of Neisseria meningitidis

Document Type

Article

Language

eng

Format of Original

5 p.

Publication Date

3-1962

Publisher

American Society for Microbiology

Source Publication

Journal of Bacteriology

Source ISSN

0021-9193

Original Item ID

DOI:10.1128/jb.83.3.470-474.1962

Abstract

Catlin, B. Wesley (Marquette University, Milwaukee, Wis.) and Gertrude M. Schloer. A defined agar medium for genetic transformation of Neisseria meningitidis. J. Bacteriol. 83:470–474. 1962.—An agar medium was developed for use in quantitative genetic studies of Neisseria meningitidis strain 15. It contains eight inorganic salts, sodium citrate, sodium lactate, arginine, cysteine, glycine, sodium glutamate, and purified agar. Abundant surface growth in the absence of supplemental carbon dioxide was obtained during 50 serial subcultures. A close correspondence was found between numbers of parental type colonies developing on the defined medium and on a complex medium. Cells subcultured serially three or four times on defined agar medium and placed directly into a solution of transforming deoxyribonucleic acid in defined liquid medium were susceptible to transformation without additional supplements. Of the treated population, 0.1 to 0.3% of the cells were transformed to streptomycin resistance.

Comments

Published version. Journal of bacteriology, Vol. 83, No. 3 (March 1962): 470-474. DOI. © 1962 American Society for Microbiology. Used with permission.

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