Document Type
Article
Language
eng
Publication Date
12-2016
Publisher
Elsevier
Source Publication
Neuroepigenetics
Source ISSN
2214-7845
Original Item ID
DOI: 10.1016/j.nepig.2016.10.002
Abstract
Axon regeneration in adult central nervous system (CNS) is limited in part by a developmental decline in the ability of injured neurons to re-express needed regeneration associated genes (RAGs). Adult CNS neurons may lack appropriate pro-regenerative transcription factors, or may display chromatin structure that restricts transcriptional access to RAGs. Here we performed epigenetic profiling around the promoter regions of key RAGs, and found progressive restriction across a time course of cortical maturation. These data identify a potential intrinsic constraint to axon growth in adult CNS neurons. Neurite outgrowth from cultured postnatal cortical neurons, however, proved insensitive to treatments that improve axon growth in other cell types, including combinatorial overexpression of AP1 factors, overexpression of histone acetyltransferases, and pharmacological inhibitors of histone deacetylases. This insensitivity could be due to intermediate chromatin closure at the time of culture, and highlights important differences in cell culture models used to test potential pro-regenerative interventions.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Venkatesh, Ishwariya; Simpson, Matthew T.; Coley, Denise M.; and Blackmore, Murray G., "Epigenetic Profiling Reveals a Developmental Decrease in Promoter Accessibility During Cortical Maturation in vivo" (2016). Biomedical Sciences Faculty Research and Publications. 166.
https://epublications.marquette.edu/biomedsci_fac/166
Comments
Published version. Neuroepigenetics, Vol. 8 (December 2016): 19-26. DOI. © 2016 The Authors. Used with permission.