Detection and Mapping of Quantitative Trait Loci that Determine Responsiveness

Authors

Janet L. Mueller, University of Illinois College of Medicine
E. A. Ellenberger, University of Illinois College of Medicine
Linda K. Vaughn, Marquette UniversityFollow
John K. Belknap, Oregon Health & Science University
Raymond M. Quock, University of Illinois College of MedicineFollow

Document Type

Article

Language

eng

Format of Original

7 p.

Publication Date

2004

Publisher

Elsevier

Source Publication

Neuroscience

Source ISSN

0306-4522

Original Item ID

doi: 10.1016/j.neuroscience.2003.10.020

Abstract

Exposure to 70% N₂O evokes a robust antinociceptive effect in C57BL/6 (B6) but not in DBA/2 (D2) inbred mice. This study was conducted to identify quantitative trait loci (QTL) in the mouse genome that might determine responsiveness to N₂O. Offspring from the F₂ generation bred from B6 and D2 progenitors exhibited a broad range of responsiveness to N₂O antinociception as determined by the acetic acid-induced abdominal constriction test. QTL analysis was then used to dissect this continuous trait distribution into component loci, and to map them to broad chromosomal regions. To this end, 24 spleens were collected from each of the following four groups: male and female F₂ mice responding to 70% N₂O in oxygen with 100% response (high-responders); and male and female F₂ mice responding with 0% response (low-responders). Genomic DNA was extracted from the spleens and genotyped with simple sequence length polymorphism MapPairs markers. Findings were combined with findings from the earlier QTL analysis from BXD recombinant inbred mice [Brain Res 725 (1996) 23]. Combined results revealed two significant QTL that influence responsiveness to nitrous oxide on proximal chromosome 2 and distal chromosome 5, and one suggestive QTL on midchromosome 18. The chromosome 2 QTL was evident only in males. A significant interaction was found between a locus on chromosome 6 and another on chromosome 13 with a substantial effect on N₂O antinociception.

Comments

Accepted version. Neuroscience, Vol. 123, No. 3 (2004): 743-749. DOI. © 2004 Elsevier B.V. Used with permission.

Recommended Citation

Mueller, Janet L.; Ellenberger, E. A.; Vaughn, Linda K.; Belknap, John K.; and Quock, Raymond M., "Detection and Mapping of Quantitative Trait Loci that Determine Responsiveness" (2004). Biomedical Sciences Faculty Research and Publications. 80.
https://epublications.marquette.edu/biomedsci_fac/80