Document Type

Article

Language

eng

Format of Original

7 p.

Publication Date

9-15-2014

Publisher

Elsevier

Source Publication

Biosensors and Bioelectronics

Source ISSN

0956-5663

Original Item ID

doi: 10.1016/j.bios.2014.03.022

Abstract

In this report, a paper-based micro-calorimetric biochemical detection method is presented. Calorimetric detection of biochemical reactions is demonstrated as an extension of current colorimetric and electrochemical detection mechanisms of paper-based biochemical analytical systems. Reaction and/or binding temperature of glucose/glucose oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calorimeter. Commercially available glucose calibration samples of 0.05, 0.15 and 0.3% wt/vol concentration are used for comparing the device performance with a commercially available glucose meter (electrochemical detection). The calorimetric glucose detection demonstrates a measurement error less than 2%. The calorimetric detection results of DNA concentrations from 0.9 to 7.3 mg/mL and temperature changes in biotin and streptavidin reaction are presented to demonstrate the feasibility of integrating the calorimetric detection method with paper based microfluidic devices.

Comments

Accepted version. Biosensors and Bioelectronics, Vol. 59 (September 15, 2014): 120-126. DOI. © 2014 Elsevier. Used with permission.

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