Date of Award

Summer 1985

Document Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Chemistry

First Advisor

Collins, Joseph M.

Second Advisor

Mendelson, Kenneth S.

Third Advisor

Feldott, Jeanette

Abstract

X-ray diffraction was used to determine the bilayer swelling properties both in water and in 30mM caC12 solution for a 3-component lipid system, composed of DOPC, DOPE and cerebroside at 10:10:7 molar ratio. The mixed lipid showed a single lamellar phase both in water and in 30mM CaC12 at room temperature. A transition from the gel to liquid crystalline phase was observed at high concentration of lipid (about 85% lipid) in water. We observed that at full hydration this lipid mixture behaves like DOPC alone. The force behavior for this mixed lipid, both in water and in 30mM CaC12 has been discussed qualitatively. The van der Waals force acting between bilayers for this mixed lipid system was comparatively weaker than that which was observed for cerebroside/DOPC systems. In 30mM caC12 , the electrostatic repulsive force between bilayers of this mixed lipid was present, and it increased both bilayer repeat spacing, d, and bilayer separation, dw. The rate of increase of d spacing with decreasing pressure was slower than what Lis et al.[ll] observed for DOPC alone in 30mM CaCl2 . Thus, we infer that the resulting electrostatic repulsion was weakened, relative to Dope in 30mM caC12.

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