Date of Award

Spring 1922

Degree Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Waring, Gail

Second Advisor

Kumaran, Krishna A.

Third Advisor

Unsworth, Brial

Abstract

The Drosophila eggshell (vitelline membrane and chorion) is a multilayered structure whose morphogenesis and function depend upon the ordered. sequential expression of approximately 30 structural genes. Vitelline membrane proteins are synthesized by follicle cells during the vitellogenic stages (8-10) along with the yolk polypeptides. The follicle cells then switch to the production of the chorionic sublayers. We are interested in deciphering the molecular signals involved in the expression of the eggshell genes. Our lab has isolated and characterized structurally several eggshell genes expressed during the stages of vitelline membrane formation. My focus is to study 5' cisacting sequences involved in the proper tissue and stage specific expression pf one of the major vitelline membrane structural genes, the sV23 gene. Preliminary P element mediated germline transformation experiments have shown that. a construct containing as little as 147 bp of 5' flanking DNA along with the coding region and 2 kb of 3' flanking DNA is sufficient for proper tissue and temporal expression. To more precisely define the 5' cis-acting sequences involved in developmental regulation. three 10 bp clustered substitutions in the 5' 147 bp region have been constructed. The effect of each mutation on sV23 gene function can then be tested by introducing the mutant constructs into sV23 null mutants. using P element mediated germline transformation.

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